Water with all its multiple uses plays a pivotal role in the sustenance of rural people, especially the poor. As such, the provision of water which go beyond domestic to include water for productive uses can enhance peoples’ livelihood options by making significant contribution to household income, food security, improved nutrition and health. All these multiple benefits, if combined can assist in the fight against hunger and poverty. This study was conducted in Mashonaland East province, covering Marondera, Murehwa and Uzumba Maramba Pfungwe districts in Zimbabwe for the period December 2005 to May 2006 to assess factors which affect multiple uses of water and their impact on the sustainability of rural water supply sources. Methodology for participatory assessment was used for data collection. The survey found that people indeed require water for productive purposes apart from domestic uses, which are often given top priority. The study found out that multiple uses of water at household level can be affected by segmentation of water services into domestic and productive water supply schemes, technology and system design, water quality and quantity and distance to water sources among other factors. The study recommends that water providers to be able to provide appropriate, efficient and sustainable services, they should understand and appreciate the livelihood needs and priorities of the communities they serve. This calls for the need for harmonization and coordination of water service providers to best respond to communities’ multiple water demands.